منابع مشابه
Genome-scale CRISPR pooled screens.
Genome editing technologies such as clustered regularly interspaced short palindromic repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The easy programmability of CRISPR using short oligonucleotides enables rapid synthesis of large-scale libraries for functional genetic screens. Here we present fundamental concepts and methods for pooled CRISPR screens and review...
متن کاملGenetic screens in vivo using the CRISPR / Cas 9 system
An unmet and paramount need in the field of cancer research is to rapidly translate basic biological findings to clinically relevant therapeutics for cancer patients. Recent technological advances have generated many innovative applications to cancer biology and in a short time have yielded a wealth of information about putative vulnerabilities across a range of cancers. The proposed work invol...
متن کاملDecoding directional genetic dependencies through orthogonal CRISPR/Cas screens
1. Department of Microbiology and Immunology; Diabetes Center at UCSF, WM Keck Center for Noncoding RNAs, University of California, San Francisco, San Francisco, California, USA. 2. Institute for Neurodegenerative Diseases, Department of Biochemistry and Biophysics, University of California, San Francisco, and Chan-Zuckerberg Biohub, San Francisco, California, USA. 3. Helen Diller Family Compre...
متن کاملCRISPR-Cas9 for medical genetic screens: applications and future perspectives.
CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR associated nuclease 9) systems have emerged as versatile and convenient (epi)genome editing tools and have become an important player in medical genetic research. CRISPR-Cas9 and its variants such as catalytically inactivated Cas9 (dead Cas9, dCas9) and scaffold-incorporating single guide sgRNA (scRNA) have been appli...
متن کاملGenomeCRISPR - a database for high-throughput CRISPR/Cas9 screens
Over the past years, CRISPR/Cas9 mediated genome editing has developed into a powerful tool for modifying genomes in various organisms. In high-throughput screens, CRISPR/Cas9 mediated gene perturbations can be used for the systematic functional analysis of whole genomes. Discoveries from such screens provide a wealth of knowledge about gene to phenotype relationships in various biological mode...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Reviews Genetics
سال: 2019
ISSN: 1471-0056,1471-0064
DOI: 10.1038/s41576-019-0192-5